Analysis Note

ControlBreast carcinoma tissue

Application

Research CategoryCell Structure

Western Blot: 10 µg/mL, membrane preparations; MD-MB-231 cells pretreated with 1-5 µg/mL ConA for 24-48 hours as positive control. 60-65 kDa proteins are identified.


When tested against recombinant MT1-MMP (lacking the TM domain) expressed in CHO cells the antibody reacts with a band of ~59 kDa.


Immunohistochemistry: Frozen tissue sections; traditional formalin fixation is problematic. Paraffin-embedded tissue sections with PLP fixation required.


Immunoprecipitation


EIA


Optimal working dilutions must be determined by the end user.

Anti-MMP-14 Antibody, hemopexin domain, clone 113-5B7 is an antibody against MMP-14 for use in ELISA, IH(P) & WB.

Research Sub CategoryMMPs & TIMPs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Manufactured by Daiichi Fine Chemical Co., Ltd

Immunogen

Epitope: a.a. 319-333

CDGNFDTVAMLRGEM in the hemopexin-like domain of human MT1-MMP

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified immunoglobulin. Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.

Specificity

Reacts with human MMP-14, also known as MT1-MMP, with a very slight cross-reactivity to hMMP-3. For unknown reasons this antibody does not react on westerns to CC1043.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

~59-69 kDa